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human cardiac fibroblast cells  (PromoCell)


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    PromoCell human cardiac fibroblast cells
    Human Cardiac Fibroblast Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 206 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human cardiac fibroblast cells/product/PromoCell
    Average 97 stars, based on 206 article reviews
    human cardiac fibroblast cells - by Bioz Stars, 2026-04
    97/100 stars

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    (A) Flowchart of conditioned media and cytokine array experiment. (B) Survival rate of <t>iCMs,</t> <t>cultured</t> in control medium (DMEM), <t>HCF</t> conditioned medium, or H 2 O 2 -treated HCF conditioned medium, after erastin treatment, normalized to respective DMSO control groups. (C) Cytokine-chemokine protein array blotting image of conditioned media from vehicle- or H 2 O 2 -treated HCFs. (D) Blotting signal intensity of IL-8 and EGF. (E, F) Survival rate of iCMs after erastin treatment in the presence of IL-8 (E) or EGF (F), normalized to DMSO control groups. (G) qPCR of CXCR1 and CXCR2 in total blood cells and purified cardiomyocyte nuclei after P1 LAD-O. Error bars indicate SD. *, p<0.05; **, p<0.01. NS, not significant.
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    (A) Flowchart of conditioned media and cytokine array experiment. (B) Survival rate of iCMs, cultured in control medium (DMEM), HCF conditioned medium, or H 2 O 2 -treated HCF conditioned medium, after erastin treatment, normalized to respective DMSO control groups. (C) Cytokine-chemokine protein array blotting image of conditioned media from vehicle- or H 2 O 2 -treated HCFs. (D) Blotting signal intensity of IL-8 and EGF. (E, F) Survival rate of iCMs after erastin treatment in the presence of IL-8 (E) or EGF (F), normalized to DMSO control groups. (G) qPCR of CXCR1 and CXCR2 in total blood cells and purified cardiomyocyte nuclei after P1 LAD-O. Error bars indicate SD. *, p<0.05; **, p<0.01. NS, not significant.

    Journal: bioRxiv

    Article Title: Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury

    doi: 10.1101/2023.02.07.527364

    Figure Lengend Snippet: (A) Flowchart of conditioned media and cytokine array experiment. (B) Survival rate of iCMs, cultured in control medium (DMEM), HCF conditioned medium, or H 2 O 2 -treated HCF conditioned medium, after erastin treatment, normalized to respective DMSO control groups. (C) Cytokine-chemokine protein array blotting image of conditioned media from vehicle- or H 2 O 2 -treated HCFs. (D) Blotting signal intensity of IL-8 and EGF. (E, F) Survival rate of iCMs after erastin treatment in the presence of IL-8 (E) or EGF (F), normalized to DMSO control groups. (G) qPCR of CXCR1 and CXCR2 in total blood cells and purified cardiomyocyte nuclei after P1 LAD-O. Error bars indicate SD. *, p<0.05; **, p<0.01. NS, not significant.

    Article Snippet: HCF cells (Cell Applications Inc, 306V-05a) were cultured in HCF Growth Medium (Cell Applications Inc, 316-500).

    Techniques: Cell Culture, Control, Protein Array, Purification

    (A, B) Wild type mouse heart tissue stained for Fth1 (magenta, A) or Ftl (magenta, B), with cTnT (green) and DAPI (blue) at 1DPMI after P7 LAD-O. Arrows, non-cardiomyocytes positive for Fth1 (A) or Ftl (B). (C, D) Mouse heart tissue stained for Fth1 (red, C) or Ftl (red, D), with Pdgfrα (grey), cTnT (green) and DAPI (blue) at 1 DPMI after P7 LAD-O. Arrows, cells positive for Pdgfrα and Fth1 (C) or Ftl (D). (E, F) Mouse heart tissue stained for Fth1 (green, E) or Ftl (green, F), with Pdgfrα (red), MF20 (grey) and DAPI (blue) at 6 DPMI after P7 LAD-O. Arrows, cells positive for Pdgfrα and Fth1 (E) or Ftl (F). (G) Diagram of cardiomyocyte-fibroblast interaction after MI. (H, I) Mouse heart section stained for Cx45 (green, H) or Cx43 (green, I) with Pdgfrα (red), MF20 (grey) and DAPI (blue) after P7 LAD-O. Arrows, potential locations of gap junctions between cardiomyocytes and fibroblasts. (J-L) Co-cultured iCM and HCF stained for VIMENTIN (VIM, Grey), free Fe 2+ (red), DAPI (blue), and imaged with TITIN-GFP (green) after DMSO (J) or erastin (15 μM) (K, L) treatment. Asterisks, HCFs with accumulation of Fe 2+ . (M) siRNA knockdown of CX43 and CX45 simultaneously in iCM-HCF co-culture, Fe 2+ fluorescent intensity ratio of iCMs over HCFs was quantified after erastin or DMSO treatment. LV, left ventricle. Error bars indicate SD. ***, p<0.001; ****, p<0.0001. Scale bar, 75 μm (A, B, E, F, H, I), 25 μm (C, D, J-L). See also Figure S4 and S5.

    Journal: bioRxiv

    Article Title: Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury

    doi: 10.1101/2023.02.07.527364

    Figure Lengend Snippet: (A, B) Wild type mouse heart tissue stained for Fth1 (magenta, A) or Ftl (magenta, B), with cTnT (green) and DAPI (blue) at 1DPMI after P7 LAD-O. Arrows, non-cardiomyocytes positive for Fth1 (A) or Ftl (B). (C, D) Mouse heart tissue stained for Fth1 (red, C) or Ftl (red, D), with Pdgfrα (grey), cTnT (green) and DAPI (blue) at 1 DPMI after P7 LAD-O. Arrows, cells positive for Pdgfrα and Fth1 (C) or Ftl (D). (E, F) Mouse heart tissue stained for Fth1 (green, E) or Ftl (green, F), with Pdgfrα (red), MF20 (grey) and DAPI (blue) at 6 DPMI after P7 LAD-O. Arrows, cells positive for Pdgfrα and Fth1 (E) or Ftl (F). (G) Diagram of cardiomyocyte-fibroblast interaction after MI. (H, I) Mouse heart section stained for Cx45 (green, H) or Cx43 (green, I) with Pdgfrα (red), MF20 (grey) and DAPI (blue) after P7 LAD-O. Arrows, potential locations of gap junctions between cardiomyocytes and fibroblasts. (J-L) Co-cultured iCM and HCF stained for VIMENTIN (VIM, Grey), free Fe 2+ (red), DAPI (blue), and imaged with TITIN-GFP (green) after DMSO (J) or erastin (15 μM) (K, L) treatment. Asterisks, HCFs with accumulation of Fe 2+ . (M) siRNA knockdown of CX43 and CX45 simultaneously in iCM-HCF co-culture, Fe 2+ fluorescent intensity ratio of iCMs over HCFs was quantified after erastin or DMSO treatment. LV, left ventricle. Error bars indicate SD. ***, p<0.001; ****, p<0.0001. Scale bar, 75 μm (A, B, E, F, H, I), 25 μm (C, D, J-L). See also Figure S4 and S5.

    Article Snippet: HCF cells (Cell Applications Inc, 306V-05a) were cultured in HCF Growth Medium (Cell Applications Inc, 316-500).

    Techniques: Staining, Cell Culture, Knockdown, Co-Culture Assay